The HSV1 DNA Quantitation (QT) Real-Time PCR kit coded HSV1DNAQT.CE is intended for the quantitative detection of Human herpes simplex virus 1 DNA in human plasma and CSF (cerebrospinal fluid) with a simultaneous control of the extraction/amplification reaction through an Internal Control (IC). The kit has been adapted for the use on the Real-Time Thermacyclers and ABI 7500 Sequence Detection System® (Software SDS version 1.3.1, Applied Biosystems™*), MX3000P (Software MxPro version 4.01, Stratagene™***) and CFX96 (Software CFX manager version 1.7, Biorad™**).
HSV-1 is a member of the Alphaherpesvirinae subfamily. Herpes simplex virus (HSV) infections are very common; the seroprevalence in adults is about 80 to 90% for HSV-1. HSV infection in immunocompetent individuals normally does not cause major health problems. HSV-1 is commonly associated with herpes outbreaks of the face known as cold sores or fever blisters. HSV reactivation in the central nervous system can occur and might cause a wide range of clinical symptoms from mild meningitis (Mollaret’s) to severe encephalitis with a mortality rate of up to 70% in the absence of therapy. HSV-1 and HSV-2 differ in their behaviour in the central nervous system (CNS). HSV-1 reactivation is the most frequent cause of viral encephalitis, a disease with a dire prognosis in the absent of early specific therapy. Primary infection of neonates or reactivation of HSV in immunocompromised individuals can be associated with a higher incidence of meningitis, severe encephalitis, and dangerous eye infections. In addition, patients with atopic eczema are prone to widespread cutaneous herpes simplex infection.
RINCIPIO DEL TEST
The HSV1DNAQT.CE Kit is based on a Real Time chemistry which uses specific Primers and Probes.
HSV1 DNA, recovered from the biological sample under investigation through an extraction step, and amplified using Real Time amplification system. The amplified product, is detected and quantified, against the standard curve using a fluorescent reporter dye probe specific for a HSV1 unique genomic sequence. Heterologous Internal Control (IC) serves as an extraction/amplification control for each individually processed specimen aiming to the identification of reaction inhibitors.
A standard curve is supplied allowing the determination of the viral load.