The BKV DNA Quantitation Real-Time PCR kit coded BKVDNAQT.CE is intended for the quantitative detection of the BK Virus DNA in human plasma and urine with a simultaneous control of the amplification/extraction reaction through an Internal Control (IC).
NTRODUCTION
Human Polyoma BK Virus (Polyomavirus hominis 1) belongs to the genus Polyomaviridae, which are non enveloped DNA viruses containing circle double-stranded DNAof about 5 Kb in size. It was first identified in 1970 from the urine of a renal allograft recipient named BK, who developed ureteric stenosis.
BK virus infection occurs during the early childhood years and is asymptomatic or associated with fever and mild upper respiratory symptoms. Up to 90% of adults are seropositive. Transmission is believed to occur via the respiratory route but this has not be formally proven.
RINCIPLE OF THE TEST
The BKVDNAQT.CE Kit is based on a Real Time chemistry which uses specific Primers and Probes.
BKV DNA, recovered from the biological sample under investigation through an extraction step, is amplified using Real Time amplification system. The amplified product is detected and quantified, against the standard curve using a fluorescent reporter dye probe specific for a BKV unique genomic sequence.
Internal Control (IC) serves as an amplification/extraction control for each individually processed specimen aiming to the identification of reaction inhibitors.
An external standard curve is supplied allowing the determination of the viral load.
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