The EBV DNA Quantitation (QT) Real-Time PCR kit coded EBVDNAQT.CE is intended for the quantitative detection of Epstein-Barr Virus DNA in human plasma and whole blood collected in EDTA with a simultaneous control of the extraction/amplification reaction through an Internal Control (IC).
EBVDNAQT..CE assay was standardised against the 1st WHO International Standard for EBV (NIBSC code 09/260) to express samples concentration also in International Unit (IU/ml).
NTRODUCTION
Epstein-Barr virus (EBV) is a common γ herpesvirus that infects more than 90% of the world’s population, leaving the majority of individuals with a lifelong silent infection. Although most primary EBV infections are asymptomatic, the virus, mainly in immunocompromised individuals such as transplant recipients and AIDS patients, is the major predisposing factor for the development of a wide range of B-cell lymphoproliferative disorders (Burkitt’s lymphoma, nasopharyngeal carcinoma, and Hodgkin’s and non-Hodgkin’s lymphomas).
The EBV genome is a linear, double stranded DNA molecule of about 175 kilobase pairs. b. It encoded a series of products interacting with or exhibiting homology to a wide variety of antiapoptotic molecules, cytokines, and signal transducers, hence promoting EBV infection, immortalization, and transformation.
RINCIPLE OF THE TEST
The EBVDNAQT.CE Kit is based on a Real Time chemistry which uses specific Primers and Probes.
EBV DNA, recovered from the biological sample under investigation through an extraction step, and amplified using Real Time amplification system. The amplified product is detected and quantified, against the standard curve using a fluorescent reporter dye probe specific for a EBV unique genomic sequence.
Heterologous Internal Control (IC) serves as an extraction/amplification control for each individually processed specimen aiming to the identification of reaction inhibitors.
A standard curve is supplied allowing the determination of the viral load.
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