The Legionella Pneumophila Qualitative Real-Time PCR kit coded LEPDNA.CE is intended for the qualitative detection of Legionella Pneumophila DNA in biological samples with a simultaneous control of the amplification reaction through an Internal Control (IC).
The kit has been adapted for the use on the Real-Time Thermacyclers and ABI 7500 Sequence Detection System® (Software SDS version 1.3.1, Applied Biosystems™*) or MX3000P (Software MxPro version 4.01, Stratagene™***).
NTRODUCTION
Legionella pneumophila, a gram negative non spore forming motile bacillus, is the etiologic agent of Legionellosis. Clinical symphtoms can range in severity from a mild, febrile illness (Pontiac fever) to a rapid and potentially fatal pneumonia (Legionnaires’ disease). L. pneumophila is a common cause of nosocomial and travel-acquired pneumonia and with the bacterial species Mycoplasma pneumoniae and Clamydia pneumoniae is one of the top three cause of sporadic, community acquired pneumonia. The first strain of legionella were isolated in 1943 and classified as Riccktesia like organism, and the genus was established in 1979 after a large outbreak of pneumonia among members of the American legion that occurred 3 years earlier. After this, a large spectrum of legionella species was classified, actually genus includes 50 species and 16 serogrups of L. pneumophila were classified. Legionellae are ubiquitous in natural and artificial water environments worldwide, and survive in a range of environmental conditions.
RINCIPLE OF THE TEST
The LEPDNA.CE Kit is based on a Real Time chemistry which uses specific Primers and Probes.
Legionella pneumophila DNA, recovered from the biological sample under investigation through an extraction step, is amplified using Real Time amplification system. The amplified product is detected using a fluorescent reporter dye probe specific for a Legionella pneumophila.
Heterologous Internal Control (IC) serves as an Extraction/Amplification control for each individually processed specimen aiming to the identification of reaction inhibitors.
An High Positive control (CTRL-H) and a Low Positive control
(CTRL-L) are supplied as controls of the PCR reaction.
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